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cloning grasp55 gfp  (Addgene inc)


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    Structured Review

    Addgene inc cloning grasp55 gfp
    Cloning Grasp55 Gfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pegfp+n1+grasp55/pEGFP-N1-GRASP55+(Plasmid+%23137708)/pmc12208438-256-20-35
    Average 93 stars, based on 3 article reviews
    cloning grasp55 gfp - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Construct:

    Article Title: SARS-CoV-2 remodels the Golgi apparatus to facilitate viral assembly and secretion
    Article Snippet: .. All pCAG plasmids encoding Strep-tagged SARS-CoV-2 proteins were kind gifts from Dr. Nevan Krogan [ ]. pHAGE2-pEF1α-GRASP55-GFP was constructed by cloning GRASP55-GFP from pEGFP-N1-GRASP55 into the pHAGE2- pEF1α vector. psPAX2 and pMD2G were obtained from Addgene. ..

    Article Title: SARS-CoV-2 remodels the Golgi apparatus to facilitate viral assembly and secretion.
    Article Snippet: .. All pCAG plasmids encoding Strep-tagged SARS-CoV-2 proteins were kind gifts from Dr. Nevan Krogan [5]. pHAGE2pEF1α-GRASP55-GFP was constructed by cloning GRASP55-GFP from pEGFP-N1-GRASP55 into the pHAGE2- pEF1α vector. psPAX2 and pMD2G were obtained from Addgene. ..

    Cloning:

    Article Title: SARS-CoV-2 remodels the Golgi apparatus to facilitate viral assembly and secretion
    Article Snippet: .. All pCAG plasmids encoding Strep-tagged SARS-CoV-2 proteins were kind gifts from Dr. Nevan Krogan [ ]. pHAGE2-pEF1α-GRASP55-GFP was constructed by cloning GRASP55-GFP from pEGFP-N1-GRASP55 into the pHAGE2- pEF1α vector. psPAX2 and pMD2G were obtained from Addgene. ..

    Article Title: SARS-CoV-2 remodels the Golgi apparatus to facilitate viral assembly and secretion.
    Article Snippet: .. All pCAG plasmids encoding Strep-tagged SARS-CoV-2 proteins were kind gifts from Dr. Nevan Krogan [5]. pHAGE2pEF1α-GRASP55-GFP was constructed by cloning GRASP55-GFP from pEGFP-N1-GRASP55 into the pHAGE2- pEF1α vector. psPAX2 and pMD2G were obtained from Addgene. ..

    RNA Extraction:

    Article Title: Ceramide-1-phosphate is a regulator of Golgi structure and is co-opted by the obligate intracellular bacterial pathogen Anaplasma phagocytophilum
    Article Snippet: The siRNAs were transfected into HUVECs using Dharmafect 4 transfection reagent (Dharmacon) per the manufacturer’s instruction. .. The cells were harvested after 48 h for RNA extraction and after 72 h for Western immunoblotting and lipidomic analyses. pEGFP-VSVGtsO45 was a gift from Dr. Jennifer Lippincott-Schwartz (Addgene plasmid #11912; http://n2t.net/addgene:11912 ; RRID:Addgene_11912). pEGFP-N1-GRASP55 (Addgene plasmid #137708; http://n2t.net/addgene:137708 ; RRID:Addgene_137708), and pEGFP-N2-GRASP65 (Addgene plasmid #137709; http://n2t.net/addgene:137709 ; RRID:Addgene_137709) were gifts from Dr. Yanzhuang Wang. .. Lipofectamine 3000 (Invitrogen) and Lipofectamine LTX (Invitrogen) were used to transfect RF/6A cells and HUVECs, respectively.

    Article Title: Ceramide-1-phosphate is a regulator of Golgi structure and is co-opted by the obligate intracellular bacterial pathogen Anaplasma phagocytophilum .
    Article Snippet: The siRNAs were transfected into HUVECs using Dharmafect 4 transfection reagent (Dharmacon) per the manufacturer’s instruction. .. The cells were harvested after 48 h for RNA extraction and after 72 h for Western immunoblotting and lipidomic analyses. pEGFP-VSVGtsO45 was a gift from Dr. Jennifer Lippincott-Schwartz (Addgene plasmid #11912; http://n2t.net/addgene:11912; RRID:Addgene_11912). pEGFP-N1-GRASP55 (Addgene plasmid #137708; http://n2t.net/ addgene:137708; RRID:Addgene_137708), and pEGFP-N2-GRASP65 (Addgene plasmid #137709; http://n2t.net/addgene:137709; RRID:Addgene_137709) were gifts from Dr. Yanzhuang Wang. .. Lipofectamine 3000 (Invitrogen) and Lipofectamine LTX (Invitrogen) were used to transfect RF/6A cells and HUVECs, respectively.

    Western Blot:

    Article Title: Ceramide-1-phosphate is a regulator of Golgi structure and is co-opted by the obligate intracellular bacterial pathogen Anaplasma phagocytophilum
    Article Snippet: The siRNAs were transfected into HUVECs using Dharmafect 4 transfection reagent (Dharmacon) per the manufacturer’s instruction. .. The cells were harvested after 48 h for RNA extraction and after 72 h for Western immunoblotting and lipidomic analyses. pEGFP-VSVGtsO45 was a gift from Dr. Jennifer Lippincott-Schwartz (Addgene plasmid #11912; http://n2t.net/addgene:11912 ; RRID:Addgene_11912). pEGFP-N1-GRASP55 (Addgene plasmid #137708; http://n2t.net/addgene:137708 ; RRID:Addgene_137708), and pEGFP-N2-GRASP65 (Addgene plasmid #137709; http://n2t.net/addgene:137709 ; RRID:Addgene_137709) were gifts from Dr. Yanzhuang Wang. .. Lipofectamine 3000 (Invitrogen) and Lipofectamine LTX (Invitrogen) were used to transfect RF/6A cells and HUVECs, respectively.

    Article Title: Ceramide-1-phosphate is a regulator of Golgi structure and is co-opted by the obligate intracellular bacterial pathogen Anaplasma phagocytophilum .
    Article Snippet: The siRNAs were transfected into HUVECs using Dharmafect 4 transfection reagent (Dharmacon) per the manufacturer’s instruction. .. The cells were harvested after 48 h for RNA extraction and after 72 h for Western immunoblotting and lipidomic analyses. pEGFP-VSVGtsO45 was a gift from Dr. Jennifer Lippincott-Schwartz (Addgene plasmid #11912; http://n2t.net/addgene:11912; RRID:Addgene_11912). pEGFP-N1-GRASP55 (Addgene plasmid #137708; http://n2t.net/ addgene:137708; RRID:Addgene_137708), and pEGFP-N2-GRASP65 (Addgene plasmid #137709; http://n2t.net/addgene:137709; RRID:Addgene_137709) were gifts from Dr. Yanzhuang Wang. .. Lipofectamine 3000 (Invitrogen) and Lipofectamine LTX (Invitrogen) were used to transfect RF/6A cells and HUVECs, respectively.

    Plasmid Preparation:

    Article Title: Ceramide-1-phosphate is a regulator of Golgi structure and is co-opted by the obligate intracellular bacterial pathogen Anaplasma phagocytophilum
    Article Snippet: The siRNAs were transfected into HUVECs using Dharmafect 4 transfection reagent (Dharmacon) per the manufacturer’s instruction. .. The cells were harvested after 48 h for RNA extraction and after 72 h for Western immunoblotting and lipidomic analyses. pEGFP-VSVGtsO45 was a gift from Dr. Jennifer Lippincott-Schwartz (Addgene plasmid #11912; http://n2t.net/addgene:11912 ; RRID:Addgene_11912). pEGFP-N1-GRASP55 (Addgene plasmid #137708; http://n2t.net/addgene:137708 ; RRID:Addgene_137708), and pEGFP-N2-GRASP65 (Addgene plasmid #137709; http://n2t.net/addgene:137709 ; RRID:Addgene_137709) were gifts from Dr. Yanzhuang Wang. .. Lipofectamine 3000 (Invitrogen) and Lipofectamine LTX (Invitrogen) were used to transfect RF/6A cells and HUVECs, respectively.

    Article Title: Ceramide-1-phosphate is a regulator of Golgi structure and is co-opted by the obligate intracellular bacterial pathogen Anaplasma phagocytophilum .
    Article Snippet: The siRNAs were transfected into HUVECs using Dharmafect 4 transfection reagent (Dharmacon) per the manufacturer’s instruction. .. The cells were harvested after 48 h for RNA extraction and after 72 h for Western immunoblotting and lipidomic analyses. pEGFP-VSVGtsO45 was a gift from Dr. Jennifer Lippincott-Schwartz (Addgene plasmid #11912; http://n2t.net/addgene:11912; RRID:Addgene_11912). pEGFP-N1-GRASP55 (Addgene plasmid #137708; http://n2t.net/ addgene:137708; RRID:Addgene_137708), and pEGFP-N2-GRASP65 (Addgene plasmid #137709; http://n2t.net/addgene:137709; RRID:Addgene_137709) were gifts from Dr. Yanzhuang Wang. .. Lipofectamine 3000 (Invitrogen) and Lipofectamine LTX (Invitrogen) were used to transfect RF/6A cells and HUVECs, respectively.



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